• Target：
• HIF-1α

• Fields：
• >>HIF-1 signaling pathway;>>Mitophagy - animal;>>Autophagy - animal;>>Th17 cell differentiation;>>Thyroid hormone signaling pathway;>>Kaposi sarcoma-associated herpesvirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Renal cell carcinoma;>>Central carbon metabolism in cancer;>>Choline metabolism in cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer

• Gene Name：
• HIF1A

• Protein Name：
• Hypoxia-inducible factor 1-alpha

• Human Gene Id：
• 3091

• Human Swiss Prot No：
• Q16665

• Mouse Gene Id：
• 15251

• Mouse Swiss Prot No：
• Q61221

• Immunogen：
• Purified recombinant fragment of human HIF-1α expressed in E. Coli.

• Specificity：
• HIF-1α Monoclonal Antibody detects endogenous levels of HIF-1α protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• HIF1A;BHLHE78;MOP1;PASD8;Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS doma

• Molecular Weight(Da)：
• 93kD

• References：
• 1. Int J Radiat Oncol Biol Phys. 2008 Dec 1;72(5):1551-9.
  2. Eur J Appl Physiol. 2009 Mar;105(4):515-24.

• Background：
• hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011],

• Function：
• domain:Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).,function:Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Acti

• Subcellular Location：
• Cytoplasm . Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602). .

• Expression：
• Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia. American Journal of Cancer Research Am J Cancer Res. 2015; 5(9): 2626–2642 WB Human HeLa cell, MDA-MB-231 cell

HIF-1α induces hypoxic apoptosis of MLO-Y4 osteocytes via JNK/caspase-3 pathway and the apoptotic-osteocyte-mediated osteoclastogenesis in vitro. TISSUE & CELL Tissue Cell. 2020 Dec;67:101402 IF Mouse 1:200 MLO-Y4 cell

Selective degradation of PL2L60 by metabolic stresses‑induced autophagy suppresses multi‑cancer growth ONCOLOGY REPORTS Lei Sun WB Human 1:500 MDA-MB-231 cell,HeLa cell

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